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Purpose To detect the expression of Cathepsin D(CTSD),heat shock protein 90α (HSP90α) and epidermal growth factor receptor (EGFR) in gastric cancer (GC) tissues and to analyze the association of their expression levels and clinical pathological features of GC,the role of CTSD,HSP90α,EGFR in the carcinogenesis,progression,invasion and metastasis of GC.Methods The expression of CTSD,HSP90α and EGFR in normal gastric mucosa,dysplasia adjacent to carcinoma,GC tissues and lymph node metastasis was measured by immunohistochemistry (IHC) and in situ hybridization (ISH).Results In 110 cases of normal gastric mucosa,83 cases of dysplasia adjacent to carcinoma,110 cases of GC and 78 cases of the lymph node metastasis,the positive rate of CTSD,HSP90α,EGFR protein was 0,18.7％,80.91％ and 92.31％,0.9％,15.66％,75.45％ and 89.74％,0,12.05％,69.09％ and 84.62％,respectively.Correlation analysis showed that the expression CTSD,HSP90α and EGFR in GC tissues was positively correlated (rs =0.853,P ＜ 0.05,rs =0.639,P＜0.05,rs =0.734,P＜0.05).Expression of CTSD,HSP90α and EGFR in GC was correlated with the degree of tumor differentiation,invasive depth,number of lymph node metastasis and TNM stages (P ＜ 0.05).There was no significant correlation with the patient's age,sex,tumor size (P ＞0.05).Conclusion CTSD,HSP90α and EGFR may be associated with malignant behavior,development and invasion and metastasis of GC.
ABSTRACT
OBJECTIVE@#To search for the differentially expressed proteins of nasopharyngeal carcinoma (NPC),and provide scientific evidence for identifying molecular biomarkers for NPC.@*METHODS@#Laser capture microdissection (LCM) was used to purify the target cells from NPC and normal nasopharyngeal epithelial tissues (NNET). Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins of microdissected NPC and NNET, PDQuest software was applied to analyze 2-DE images,and the differential proteins between the 2 types of tissues were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Western blot and immunohistochemistry of tissue microarray were used to detect the expression of the differential protein SCCA1 in NPC and NNET.@*RESULTS@#2-DE patterns of microdissected NPC and NNEC were established,and 36 differential proteins in the NPC and NNEC were identified,20 of which only expressed or up-regulated in NPC and 16 only expressed or up-regulated in NNET. The differentially expressed level of SCCA1 in the NPC and NNET was confirmed by Western blot and immunohistochemistry of tissue microarray.@*CONCLUSION@#Thirty-six differentially expressed proteins identified in this study may be associated with the carcinogenesis of NPC,and may be candidate molecular biomarkers for NPC.